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What are the various methods used to extract Polyhydroxyalkanoates from bacteria, following the fermentation process?

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1 Answers

  1. sindhuramar 2 years ago #

    There are two common methods employed for the downstream extraction of PHA from bacteria viz., solvent extraction and the enzyme cocktail extraction


    The process of solvent extraction, is based on the solubility of PHA in chloroform and insolubility in methanol. In this method, the bacterial cells are concentrated, dried and extracted with hot methanol. PHA is dissolved in warm chloroform and the residual cell debris is removed from the chloroform by solid-liquid separation process. The PHA is then precipitated by addition of methanol and recovered by solid-liquid separation process. PHA is washed with methanol to enhance the quality and dried.

    A cocktail of enzymes, including proteases, nucleases, lysozymes and detergents which are used to treat the bacterial cells in order to remove proteins, nucleic acids and cell walls, leaving the PHA intact, which could finally be recovered by the process of solid liquid separation.



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