Hello, there are various types of extraction methods, the most commonly used extraction method for pharma and food grade DHA or EPA is SC CO2 (supercritical fluid extraction). This method is highly efficient in terms of quantity and quality. The pressure and temperature depends on the combination of fatty acids. Some algae contains only EPA or DHA in higher amount and some algae contains both DHA and EPA. Trials are required to find out optimum temperature/pressure for a particular algae species. A Similar work has been carried out by University of Perugia, Italy. Here is a link to the published paper - http://www.isasf.net/fileadmin/files/Docs/Versailles/Papers/N4.pdf. This temperature and pressure given can be used as a benchmark.

Hi Narsi, sorry for the late answer, your input did not arrived in my email. No I have no ideas on which type of strains could be , however if you follow these links http://news.harvard.edu/gazette/story/2010/10/microbes-to-the-rescue/?fb_xd_fragment#?=&cb=ff4acda8f0e5b&relation=parent&transport=fragment&frame=f1b3d2a73b29dff and http://www.whoi.edu/oceanus/viewArticle.do?id=95889&sectionid=1000 we can understand that some "algae" dedicated for this scope is growing. I am located in Italy so ... however I suppose could be usefull to have some feedback from that area

I suppose it can be. Induced or assisted hyper-accumulation involves the process of adding a conditioning fluid containing a chelator or another agent to soil to increase metal solubility or mobilization so that the plants can absorb them more easily. Although, in many cases natural hyperaccumulators are metallophyte plants that can tolerate and incorporate high levels of toxic metals.

I suppose it could be said so. Phytovolatilization involves the process of uptake and transpiration of contaminants, primarily organic compounds, by plants. The contaminant, present in the water taken up by the plant, passes through the plant or is modified by the plant, and is released to the atmosphere (evaporates or vaporizes).

Bioretention is the process in which contaminants and sedimentation are removed from stormwater runoff. That makes it a significant concept in bio remediation.

Phytostimulation, also referred to as enhanced rhizosphere biodegradation, rhizodegradation, or plant-assisted bioremediation/degradation, is the breakdown of organic contaminants in the soil via enhanced microbial activity in the plant root zone or rhizosphere. This method is useful in removing organic contaminants, such as pesticides, aromatics, and polynuclear aromatic hydrocarbons (PAHs), from soil and sediments. Chlorinated solvents also have been targeted at demonstration sites.

But thats just the phase I mechanism of phytotransformation. Phase II is the stage where the plant biomolecules such as glucose and amino acids are added to the polarized xenobiotic to further increase the polarity (known as conjugation). Phase III involves the sequestration of the xenobiotic within the plant. The xenobiotics polymerize in a lignin-like manner and develop a complex structure that is sequestered in the plant. This ensures that the xenobiotic is safely stored, and does not affect the functioning of the plant. Hence, the plants reduce toxicity (with exceptions) and sequester the xenobiotics in phytotransformation.