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Latest Questions - Biotechnology

  • I am looking for which methods would be best for me to test for a science fair experiment. I want to go with something that is relatively energy efficient. I'm specifically interested in OriginOils and Solazyme's methods of extraction, or anything that hasn't been researched much but looks promising.

    I do have access to some equipment at a biochemistry lab, but I want to do as much as a highschool student could pull off at home.

    in Biodiesel Algae Fuels Oil and Cleantech Public Transportation Biopolymers and Bioplastics Biotechnology

    • View all 4 answers
    • Rcollins 8 months ago

      One cheap and easy way to extract the algae is to use a membrane material to "screen out the algae" manually. It has to be extremely fine mesh, depending on what strain of algae you are growing, (with Nannochloropsis oculata a coffee filter is too porous, just goes right through it). Then you can dry the algae, you can sun dry it, or use heat lamps, or hair drier... you could even vacuum dry it if you wanted too. One way to extract the oil after the algae is dried is to grind it up and press it.

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  • I really want to run some experiments growing algae heterotrophically or maybe mixotrophically. I have heard that cell densities as well as lipid content can get to very high levels. But I am having trouble finding good literature [technical or otherwise] to base experiments from. Could someone please help me out, or at least point me in the right direction

    in Biomass Power Production Biodiesel Algae Fuels Biotechnology

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  • hello peaple
    please answer my question
    how can we extract EPA and DHA from microalgae?
    is EPA AND DHA ON THE oils of microalgae or on the dry powders?
    if its on the powder,how can we extract it?
    please answer me

    in Biodiesel Algae Fuels Bioremediation Biotechnology

    • Parkavi 8 months ago

      Hello, there are various types of extraction methods, the most commonly used extraction method for pharma and food grade DHA or EPA is SC CO2 (supercritical fluid extraction). This method is highly efficient in terms of quantity and quality. The pressure and temperature depends on the combination of fatty acids. Some algae contains only EPA or DHA in higher amount and some algae contains both DHA and EPA. Trials are required to find out optimum temperature/pressure for a particular algae species. A Similar work has been carried out by University of Perugia, Italy. Here is a link to the published paper - http://www.isasf.net/fileadmin/files/Docs/Versailles/Papers/N4.pdf. This temperature and pressure given can be used as a benchmark.

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  • How do you see algae being incorporated into your home?

    in CO2 Sequestration Domestic Wastewater Treatment Sustainable Homes and Communities Biotechnology

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  • What do you think about it?
    http://www.smartplanet.com/blog/intelligent-energy/ecoli-bacteria-that-eats-switchgrass-to-make-fuel/11254?tag=nl.e660
    http://www.smartplanet.com/blog/smart-takes/new-e-coli-bacteria-strain-can-turn-biomass-into-biodiesel/3754?tag=search-river

    in Biomass Power Production Biodiesel Biotechnology

    • Donmichael 1 year ago

      The DEO has a patent like this for sale right now. I read about it a few months ago but we are some ways off from comercialization.

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  • Kyzyl wants to know Do you know? 1 year ago

    UMaine Researchers Discover Revolutionary Process for Biofuel. http://umaine.edu/news/blog/2011/10/24/umaine-researchers-discover-revolutionary-process-for-biofuel/

    in Biotechnology

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  • http://news.uga.edu/releases/article/uga-researchers-develop-super-yeast-that-turns-pine-into-ethanol/

    in Biotechnology

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  • Czajna wants to know title book 1 year ago

    hey :) i need a few book on topic: biofuels/biodiesel from algae. i found a lot of article about this but i can't find some book about this.
    somebody could help me?

    in Biomass Power Production Biodiesel Algae Fuels Biotechnology

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  • Has anyone ever introduced a transgene into algae that encodes T7 RNA polymerase in algae to drive expression of other additional transgenes from T7 RNA polymerase promoters? Can this be accomplished using a nuclear construct in algae to drive expression of a version of the T7 RNA polymerase containing an N-terminal or a C-terminal transit sequence or signal sequence that could target posttranslational import of the T7 RNA polymerase to the chloroplast or to the mitochondria? Could such subcellular localization of a functional T7 RNA Polymerase enzyme be used to express transgenes targeted to the chloroplast or mitochondria if those transgenes were under the control of a functional T7 RNA polymerase promoter?

    in Biotechnology

    • Algejetfuels 1 year ago

      Excellent question. The diatom I am working with WAS modified using PCR machine in late 1990's to express chemicals in the chloroplasts of the marine diatom c cryptica. It's a centric diatom so easy to modify and work with, and it's already a great oil producer. I don,t think RNA was modified at that time. Would you like to try it on our strain and see what happens? Good documentation online

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  • Needing to demonstrate Energy Balance for a novel Algae application for the usual funding hurdles. I am looking for ANY university level research demonstrating (at the plant physics level) Energy requirements for algae growth - from both light and heat sources.
    ie: energy input for a RATE of algae growth - or better still Oxygen production from Algae.
    Specifically, we know algae grows faster at temperature. What is the mechanism? What is the impact of this on need for energy from light.
    Thanks!

    in Algae Fuels Biotechnology

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