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I am looking for which methods would be best for me to test for a science fair experiment. I want to go with something that is relatively energy efficient. I'm specifically interested in OriginOils and Solazyme's methods of extraction, or anything that hasn't been researched much but looks promising.
I do have access to some equipment at a biochemistry lab, but I want to do as much as a highschool student could pull off at home.in Biodiesel Algae Fuels Oil and Cleantech Public Transportation Biopolymers and Bioplastics Biotechnology
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Joernst wants to know Algae fed-batch system 9 months ago
I really want to run some experiments growing algae heterotrophically or maybe mixotrophically. I have heard that cell densities as well as lipid content can get to very high levels. But I am having trouble finding good literature [technical or otherwise] to base experiments from. Could someone please help me out, or at least point me in the right direction
in Biomass Power Production Biodiesel Algae Fuels Biotechnology
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yeah right. i got confused. i would stick with a culture system without any carbon source than CO2.
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hello peaple
please answer my question
how can we extract EPA and DHA from microalgae?
is EPA AND DHA ON THE oils of microalgae or on the dry powders?
if its on the powder,how can we extract it?
please answer me- 1 person added this post to their reference
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Hello, there are various types of extraction methods, the most commonly used extraction method for pharma and food grade DHA or EPA is SC CO2 (supercritical fluid extraction). This method is highly efficient in terms of quantity and quality. The pressure and temperature depends on the combination of fatty acids. Some algae contains only EPA or DHA in higher amount and some algae contains both DHA and EPA. Trials are required to find out optimum temperature/pressure for a particular algae species. A Similar work has been carried out by University of Perugia, Italy. Here is a link to the published paper - http://www.isasf.net/fileadmin/files/Docs/Versailles/Papers/N4.pdf. This temperature and pressure given can be used as a benchmark.
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Joshwolf wants to know Residential Algae! 1 year ago
How do you see algae being incorporated into your home?
in CO2 Sequestration Domestic Wastewater Treatment Sustainable Homes and Communities Biotechnology
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It would be cool to have a gas pump that goes form the point of fuel processing to the garage too
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The DEO has a patent like this for sale right now. I read about it a few months ago but we are some ways off from comercialization.
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Kyzyl wants to know Do you know? 1 year ago
UMaine Researchers Discover Revolutionary Process for Biofuel. http://umaine.edu/news/blog/2011/10/24/umaine-researchers-discover-revolutionary-process-for-biofuel/
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Czajna wants to know title book 1 year ago
hey :) i need a few book on topic: biofuels/biodiesel from algae. i found a lot of article about this but i can't find some book about this.
somebody could help me?in Biomass Power Production Biodiesel Algae Fuels Biotechnology
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well, i try it. :) thx for idea :)
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Msabad wants to know Genetic engineering of chloroplasts in algae. 1 year ago
Has anyone ever introduced a transgene into algae that encodes T7 RNA polymerase in algae to drive expression of other additional transgenes from T7 RNA polymerase promoters? Can this be accomplished using a nuclear construct in algae to drive expression of a version of the T7 RNA polymerase containing an N-terminal or a C-terminal transit sequence or signal sequence that could target posttranslational import of the T7 RNA polymerase to the chloroplast or to the mitochondria? Could such subcellular localization of a functional T7 RNA Polymerase enzyme be used to express transgenes targeted to the chloroplast or mitochondria if those transgenes were under the control of a functional T7 RNA polymerase promoter?
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Excellent question. The diatom I am working with WAS modified using PCR machine in late 1990's to express chemicals in the chloroplasts of the marine diatom c cryptica. It's a centric diatom so easy to modify and work with, and it's already a great oil producer. I don,t think RNA was modified at that time. Would you like to try it on our strain and see what happens? Good documentation online
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Needing to demonstrate Energy Balance for a novel Algae application for the usual funding hurdles. I am looking for ANY university level research demonstrating (at the plant physics level) Energy requirements for algae growth - from both light and heat sources.
ie: energy input for a RATE of algae growth - or better still Oxygen production from Algae.
Specifically, we know algae grows faster at temperature. What is the mechanism? What is the impact of this on need for energy from light.
Thanks!- View all 3 answers
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This link might be of help too - CSIRO, Australia - http://www.marine.csiro.au/microalgae/index.html And here is a cool link (a downloadable PDF) that provides details of all the microalgae projects in Australia - http://www.presentations-dlgbenelux.com/Congres/download/2a65add6a94d65511aaffc84a29af448/08092011105521/572
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Latest Discussions - Biotechnology
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Parkavi wants to discuss Jiaonan to build national microalgae DHA production base 8 months ago
A microalgae DHA production project was carried out by Qingdao Langyatai (Group) Co Ltd and Qingdao Institute of Bioenergy and Bioprocess Technology, and launched in Jiaonan to extract DHA from microalgae.
It is located in Jiaonan Seaside Industrial Park covering an area of 2.67 hectare, the overall floorage of which is about 11, 940 square meters. With proprietary intellectual property rights, it is mapped out to achieve the DHA production scale of 1000-t/a and turn into the largest national microalgae DHA production base in three years.Company : Algaetech Sdn Bhd -
Sumukhi shared a video Spirulina Cultivation by Women in Madurai to Combat Malnutrition 9 months ago
This video features the low-cost cultivation of spirulina brings one answer to child malnutrition. This farm located in Madurai, State of Tamil Nadu is operated by 15 women operating 60 tanks that can produce 4 Kg of dry Spirulina/day.
This farm is owned by an NGO- Antenna technologies headquartered in Geneva, Switzerland. More details about this organisation can be found here - http://www.antenna.ch/en/- 1 person added this post to their reference
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Gaya3 wants to discuss Ohio State partner in $5.7M grant to develop new biofuel - North Texas e-News 11 months ago
Ohio State partner in $5.7M grant to develop new biofuel - North Texas e-News - http://www.ntxe-news.com/artman/publish/article_77126.shtml
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Joshwolf shared a video My Algae Biodiesel Presentation 1 year ago
I am proposing a new solution to the algae biofuel industry. Using electrical stimulation, I have created biodiesel from algae for $0.96/gallon. Come view this presentation of my project
in Biomass Power Production Biobutanol Biodiesel Algae Fuels Biomass to Liquid Batteries Biodiversity Agri Waste Management Anaerobic Digestion of Waste Bioremediation Batteries for Electric Vehicles Biopolymers and Bioplastics Biotechnology Biomimicry
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great job. do u have any paper published about it?
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Gaya3 shared a video Tobacco to Fuel 1 year ago
UC Berkley scientists talk about their research on converting carbon in tobacco to fuel.
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Gaya3 wants to discuss Genetic Engineering Made Easy 1 year ago
Life Technologies has announced the launch of GeneArt® Precision TALs, generally referred to as TAL effector proteins (TALE), which will allow... - http://www.algaeindustrymagazine.com/geneart®-precision-tals/?utm_source=feedburner
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Gaya3 wants to discuss Methyle Ketones as a New Biofuel 1 year ago
Scientists engineer the E.Coli to produce methyl ketones - high cetane numbers make them good candidates of biofuels but they have a high melting point which is an unfavorable fuel property. http://www.sciencedaily.com/releases/2012/03/120314101245.htm
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Joshwolf shared a picture MEET LEVI 1 year ago
Through extensive testing I've performed over the last 2 months, I've concluded that the method of Low Energy Voltaic Impulse(LEVI) destroys specific protein channels along the cell wall membrane, allowing for lipid bodies (and some cytoplasmic fluids) to be secreted by the cell. By pumping this device with 120 gallons of water I was able to produce 4.35 Liters of lipid bodies which converted to 4.1 Liters of diesel fuel via transesterification. To run down 2 D cell batteries, I needed to perform this test 82 times producing just over 90 gallons of diesel fuel, and the lowest cost fuel conversion price of $0.03
in Biodiesel Algae Fuels Batteries Cleantech Education in Schools Biotechnology
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Josh have you computed how many gallons of water total you would need to grow enough botryococcus for a continuous supply of ripe algae to be processed by LEVI. In other words how many gallons of water would you need to process that 90 gallons continuously?
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Arunpillai wants to discuss Harnessing nature’s solar cells 1 year ago
Andreas Mershin, a research scientist in the MIT Center for Bits and Atoms, noticed that while most of the pines had bare trunks and a canopy of branches only at the very top, a few had small branches all the way down the length of the trunk, capturing any sunlight that trickled down from above. He decided to create a microscopic forest on a chip, with PS-I coating his “trees” from top to bottom.
Turning that insight into a practical device took years of work, but in the end Mershin was able to create a tiny forest of zinc oxide (ZnO) nanowires as well as a sponge-like titanium dioxide (TiO2) nanostructure coated with the light-collecting material derived from bacteria. The nanowires not only served as a supporting structure for the material, but also as wires to carry the flow of electrons generated by the molecules down to the supporting layer of material, from which it could be connected to a circuit. “It’s like an electric nanoforest,” he says.
Read More - http://www.mit.edu/newsoffice/2012/biosolar-0203.html -
Joshwolf wants to discuss MY LEVI/OILVACUOLE FUSION PROCESS 1 year ago
Wolf Biodiesel - http://wolfbiodiesel.com/
It's very relevant to show numerous graphics in the description of how I go about lipid production through electrical impulsing so I have a link to my website where I've posted a blog about this process.
To view the page, Click on "Blog" in the top right and Select "LIPID VACUOLE FUSION AND L.E.V.I."in Biobutanol Algae Fuels Biomass to Liquid Biodiversity Water Purification Biotechnology
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Kyzyl wants to discuss To adherents of algaes application this information could be useful: Sewage-eating algae used to treat wastewater in test project | The Tribune & SanLuisObispo.com 1 year ago
Sewage-eating algae used to treat wastewater in test project | The Tribune & SanLuisObispo.com - http://www.sanluisobispo.com/2012/01/25/1922687/algae-wastewater-clean-sewage.html
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Kyzyl wants to discuss Bioengineering e.coli to turn seaweed into fuel | SmartPlanet 1 year ago
Scientists at Bio Architecture Lab tinkered with the superbug e.coli and turned it into a microbe that can turn the sugar in seaweed into fuel, and ma - http://www.smartplanet.com/blog/intelligent-energy/bioengineering-ecoli-to-turn-seaweed-into-fuel/12264?tag=nl.e660
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Joshwolf shared a picture RECYCLED ALGAE DRYING METHOD 1 year ago
This model I made uses old pop cans painted black to dry algae. Filtered algae sludge is fills the pop cans. The pop cans then sit out in the sun for 3 hours, evaporating out the water in the algae to an 8%water content. The dry meal can be used for composters, fertilizer and diesel fuel production. (Every dry kilogram of algae has sequestered 2.2 kilograms of CO2)
in Biodiesel Algae Fuels Biomass to Liquid Solar Water Heating and Drying CO2 Sequestration Biotechnology
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Josh, great job! I'm sure there is a way to make a product with this concept but I'm concerned about the time it takes to fill up the cans. At the very least this is probably the most economical method of drying I've seen done on a small scale for individual use. Do you have plans for scaling up as a waste processor from continuous production? That way you would have a continuous waste of sludge. How long did it take to get all that sludge and what is the ratio of sludge to your primary deisel production in the same amount of time? @Woolncathairs, after the algae is dried, the oil is pressed out with a screw press, and the rest is then heated out. Or... Flash pyrolysis is an energy intensive method that converts algae paste directly into bio crude which then is converted into b100 diesel through transesterfication then is cleaned if needed. Transesterfication is the most used method after pressing as well. However, the best way to get oil out of algae is through live extraction or "milking" which basically sucks the oil out of the algae without harming them. The oil then floats to the top which can then be extracted and processed, and the harvested algae sinks to the bottom where it can be taken back into the bioreactor to regrow more oil. This is way more efficient than batch processing which kills the algae. Batch processing may be good for some applications let's say if you want to extract proteins, carbohydrates and fats for multiple uses; but this is energy intensive because you have to dry the algae to get the products out which also requires various separation methods. To avoid the cost of separation and processing with batch harvesting, it is a good method to produce a high BTU biomass to burn for steam power in a coal plant (the waste heat from the plant can be used to dry the algae) but not for applications that target one needed product like oil. This is also due to the fact that a batch of algae killed requires a batch of algae born, and regrowing new fat cells is way less energy intensive than growing a whole new body. Compare it to telling a fat person to go get fat again after lyposuction; to killing a fat person and telling his friends to have a bunch of kids to grow up and replace him. If you're going to do any research at all it should be on milking oils, carbs, and proteins for specific applications at their point of use to minimize transportation cost. That is one way to undercut fossil fuel prices because fossil fuels cannot be cultivated anywhere and therefore require a large transportation infrastructure. Also, in regards to the drying method. Once you press out the oils the rest is fermented to get alcohol out of the sugars while the protein is used as feedstock and the waste from fermentation is used for fertilizers. There is a wide range of uses for that waste though instead of just fertilzers, like recycling it back into the system as algae fertilizer or used for processing, for better energy conversion.
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Kyzyl wants to discuss Evaluation of microbial biofilm communities from an Alberta oil sands tailings pond - Golby - 2011 - FEMS Microbiology Ecology - Wiley Online Library 1 year ago
Evaluation of microbial biofilm communities from an Alberta oil sands tailings pond - Golby - 2011 - FEMS Microbiology Ecology - Wiley Online Library - http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2011.01212.x/full
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I think this is an exciting area of research. As much as one would hate getting oil from oil sands, it is a reality, especially for Alberta in Canada which I believe could be an ecological Hiroshima in a few years from now. I am not sure if the microbial critters could save it from becoming one, but looks like if someone has a chance, then its them critters Good luck!
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Kyzyl wants to discuss Researchers Create Living âNeon Signsâ Composed of Millions of Glowing Bacteria 1 year ago
In an example of life imitating art, biologists and bioengineers at UC San Diego have created a living neon sign composed of millions of bacterial cells that periodically fluoresce in unison like blinking light bulbs. - http://ucsdnews.ucsd.edu/pressreleases/researchers_create_living_neon_signs_composed_of_millions_of_glowing_bacter/
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Kyzyl wants to discuss Biofuel research boosted by discovery of how cyanobacteria make energy 1 year ago
Biofuel research boosted by discovery of how cyanobacteria make energy - http://www.sciencedaily.com/releases/2011/12/111215141613.htm?utm_source=feedburner
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Kyzyl wants to discuss E. coli bacteria engineered to eat switchgrass and make transportation fuels 1 year ago
E. coli bacteria engineered to eat switchgrass and make transportation fuels - http://www.sciencedaily.com/releases/2011/11/111129123307.htm?utm_source=feedburner
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Ps21 posted a blog Sustainable Future in Development Countries (Latin America, Africa and Asia). 1 year ago
Research and Innovation in Low Cost , Green & Clean Technologies to provide food and feed to our communities and hungry people, upgrade to efficient and integrated production systems, entrepreneuships and community development, environmental protection and conservation, mitigation & adaptation to climate change. Algal Technology as a key-stone for integrated systems (water treatment, food production, CO2 capture and others) in urban and rural environments. Eolian & solar energy (chemical or photosynthetic, thermic and photovoltaic) to move production systems more efficiently.
in Solar PV Solar Thermal Sustainable Agriculture and Forestry Organic Farming Global Warming and Climate Change Biodiversity CO2 Sequestration Agri Waste Management Water Conservation and Reuse Domestic Wastewater Treatment Sustainable Homes and Communities Clean Development Mechanism Biotechnology
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Slowly but surely, the industry is embracing renewable feedstocks to replace at least some petroleum-derived processes. Those companies that don’ - http://www.smartplanet.com/blog/business-brains/report-green-chemistry-could-save-companies-655b/19825?tag=nl.e660
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Kyzyl wants to discuss Nano-tech makes medicine greener – University of Copenhagen 1 year ago
Nano-tech makes medicine greener – University of Copenhagen - http://news.ku.dk/all_news/2011/2011.11/nano-tech_makes_medicine_greener/

















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Rcollins 8
months ago
Answer this question / Share a linkOne cheap and easy way to extract the algae is to use a membrane material to "screen out the algae" manually. It has to be extremely fine mesh, depending on what strain of algae you are growing, (with Nannochloropsis oculata a coffee filter is too porous, just goes right through it). Then you can dry the algae, you can sun dry it, or use heat lamps, or hair drier... you could even vacuum dry it if you wanted too. One way to extract the oil after the algae is dried is to grind it up and press it.